[英文释义] The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
Standard guide for characterization of type i collagen as a starting material for surgical implants and substrates for tissue engineered medical products 作为组织工程医疗产品用外科植入物和底物原材料的i型胶原表征的表征指南
Pga, phb meshes were dipped into cross-linked type i collagen solution, dried under vacuum freeze condition in a special annular model . the tubular porous scaffolds have elasticity, tenacity and hydrophilic, can maintain their shapes in culture medium for long time 3cm,放置培养液中10d,可保持管腔形状,不塌陷,不变形,表明采用pga胶原直接模具成型所形成的管形支架是一种较理想的支架材料。
Using those techniques, it is possible to reconstruct vascular model in vitro whose structure and function are same as autogenous vessels . in this experiment two degradable materials were precoated with cross-linked type i collagen, moulded into tubular porous scaffold, and then seeded with ecs . using rotary cell culture technique, vascular model was constructed in vitro 本实验采用胶原与几种可降解材料相复合,构成管形支架,在体外环境下,探索构建分别含内皮细胞、平滑肌细胞及成纤维细胞的三层结构的组织工程化血管,三种细胞相互作用、相互支持,形成一个在形态和功能与正常血管近似的组织工程化血管。
Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co-cultured in vitro to construct tet . lt was frozen in liquid nitrogen with four kinds of cpa for 2 months . post-thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon . after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined . result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice . in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed . c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature 组织工程肌腱制备完成后在四种抗冻剂保护下液氮冻存2月;快速复温后植入裸鼠以修复跟腱缺损,2、4、6、8、12周后取出,观察形态学、组织学、电镜和免疫组织化学变化,短串联重复位点检测和生物力学变化。结果实验组组织工程肌腱体内植入12周后仍有肌腱细胞存活并分泌型胶原;随着时间延长,10二甲基亚砜(dmso)+棉子糖(30mmoll)+kh_2po_4(25mmoll)组线粒体空泡减少,肌腱细胞排列整齐,胶原纤维增粗并连接,抗拉强度增高。